Introducción a la HPLC: aplicación y práctica. Front Cover. Oscar Alberto Quattrocchi. Artes Gráficas Farro, – pages. 6 oct. We are always happy to assist you. Document Description. Introduccion a La HPLC Quattrocchi. Document Share. Documents Related. Results: the HPLC method described for the simultaneous determination of sulphadoxine and pyrimethamine in mL .. Quattrocchi OA, De Andrizzi SA, Laba RF. Introducción a la HPLC Aplicación y. Práctica. Buenos Aires: Artes Gráficas.
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Chromatography was carried out on HPTLC glass backed plates 10 x 10 cm and 20 x 10 cm precoated with silica gel Flayer thickness 0. Each solution was applied as bands 3 times. Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.
In patients with normal renal and hepatic function, the plasma half-life of nifedipine is about 2 h when administered as conventional capsules, and about 7 h when administered as extended-release tablets. But, I will read this book too, thanks.
Chailapakul, Talanta 79, The linear range was between 0. The United States Pharmacopeia ; U. This mixture was vortex-mixed for 1 min and then centrifuged for 10 quattricchi at rpm. The method was applied to the determination of sulfamethazine in a veterinary commercial solution. Hello people, I am starting to work with chromatography and would like to recommend some books, I would like to better understand how to predict retention times according to chosen column and mobile phase and other things.
Detection and quantification limits.
Calibration curves were constructed using peak-area ratio for the nifedipine and the internal standard carbamazepine as a function of the concentration added. Some wavelengths were tried, and nm was selected as working wavelength. The method was sensitive enough to detect a quantity as low as 0.
The oxidation of sulfamethazine at the surface of modified electrode occurs at lower potentials than that of an unmodified carbon paste electrode, and both an enhancement of the anodic peak current and a signal narrower quaattrocchi better defined with the modified electrode were observed. No other method using HPTLC was found in the literature, for the quantification of nifedipine in human serum neither in human plasma.
Therefore, the aim of this study was to develop and validate a fast, sensitive, accurate and precise method using HPTLC for quantitative determination of nifedipine in human serum, suitable for medication adherence studies. Liquid-liquid extraction yields a good recovery of nifedipine from serum.
Hello GuiHn If you are begining with hplc and you understand spanish I recommend: Precision and accuracy Quality control samples spiked at three concentrations 0. Accordingly, a method for the determination of sulfamethazine was developed using differential pulse voltammetry, at pH 11 and with an accumulation time of 3 min.
Sulfamethazine; carbon paste; ionic liquid; modified electrodes; voltammetry. Chile, Hashemi, Quattroccih 24, High performance thin layer chromatographic determination of nifedipine in human serum after liquid-liquid extraction.
The migration distance was 8 cm and the migration time 12 min. Book recommendation Basic questions from students; resources for projects and reports.
No interference was observed. A carbon paste electrode was modified with the ionic liquid 1-methyloctyl imidazolium hexafluorophosphate and it was applied for study the electrocatalytic oxidation and voltammetric determination of the drug sulfamethazine.
Introduccion a La HPLC Quattrocchi
R f for nifedipine and carbamazepine were 0. Apologies for any authoring errors Sat Nov 30, 8: Complete resolution of the peaks with clear baseline separation was obtained of this way.
Wed Mar 07, 6: The Merck veterinary manual. The mobile phase consisted of chloroform: Sun Mar 18, 1: Calcium-channel blockers, as nifedipine, are considered one of several preferred antihypertensive drugs for the initial management of hypertension in patients with a high risk of developing coronary artery disease, including those with diabetes mellitus. The results are presented in Tables 1 and 2.
In total there introducfion 6 users online:: This method can be reliably applied to drug adherence evaluation.
The nifedipine concentration ranged between 0. Guzman-Vazquez de Prada, A.
Bioanalytical Method Validation, U. I’m sorry for my english, I’m Brazilian and I’m learning the language yet.
Book recommendation – Chromatography Forum
Is an excellent book for beginners, the only drawback is that is rather old. Determinations were performed at a wavelength of nm. Nifedipine is used alone or in combination with other classes of antihypertensive agents in the management of hypertension.
Precision was calculated by RSD and accuracy was measured from percentage recoveries obtained by the analysis of the quality control samples, determined by linear regression equation of peak area vs. All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License.
The developed modified electrode was characterized using cyclic voltammetry and scanning electron microscopy.
Tue Aug 17, 7: The accuracy and precision did not exceed 4.